The effect of PJ consumption by diabetic males on their HDL-associated PON1 activities.

Ten male patients with type 2 diabetes mellitus consumed 50 mL of concentrated pomegranate juice (PJ) per day (which contain 1.5 mmol of total polyphenols) for a period of 1 month, followed by a period of 4 weeks’ “wash-out.” Blood samples were collected from the patients before (0 time) and 4 weeks after PJ consumption, as well as after the “wash-out” period. The HDL fractions were isolated from the blood samples of four patients by ultracentrifugation. The HDL-associated PON1 arylesterase (A), and lactonase activities (B) were determined. The individual results, as well as the mean±SD are shown.