Figure 6. CIT3 Cells Expressing EGFP Fusion to the N- and C-termini of GLUT1 in GM and after 4 Days in SM
High-power images. Upper left: Plasma membrane targeting of EGFP-GLUT1 in GM. Lower left: Intracellular pattern of EGFP-GLUT1 signal in differentiated cells in SM. Upper right: Plasma membrane targeting of GLUT1-EGFP in GM. Lower right: Intracellular targeting of GLUT1-EGFP with perinuclear punctate distribution in cells exposed to SM. All images captured with 0.25 s exposure time and are at 40× magnification, except for the upper left image, which was optimized at 0.5 s exposure time and higher power (60×).