Salivary Protein Carbonylation Assay.

I (upper panel): A representative western blot (WB) analysis of total saliva proteins exposed to cigarette smoke. A: untreated control; B: after single puff of cigarette smoke; C: after three puffs of cigarette smoke; D: after nine puffs of cigarette smoke. I (lower panel): Average of densitometric analyses of three to five different WB assays of the same experiment. II (upper panel): A representative WB analysis of the total saliva proteins exposed to aldehydes. A: untreated control; B: acrolein content present in one cigarette (1 μmol); C: acrolein (10 μmol); D: acetaldehyde content present in one cigarette (20 μmol). II (lower panel): Average of densitometric analyses of three to five different WB assays of the same experiment. Reprinted from Figure 3 of Avezov K, et al.,⁵² with permission from Elsevier.