Figure 2. Oxidative Status and Lipid Metabolism upon Addition of Glycine- or Leucine-Rich Proteins (Fibroin or Casein, Respectively) to Cultured J774A.1 Macrophages
Quantifications were performed after cell incubation with fibroin (glycine-rich), casein (leucine-rich), or only glycine, followed by: (A) Intracellular ROS generation measured by 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA); (B) Cholesterol mass; (C) Triglyceride mass; (D) VLDL uptake, using fluorescein isothiocyanate (FITC)-labeled VLDL; (E) Triglyceride biosynthesis rate after cell incubation with [3H]-oleic acid; (F) Triglyceride degradation rate versus Control.